Genetic engineering with viruses

Genetic Engineering of Plants for Resistance to Viruses

Genetic engineering has been identified as one key approach to increasing agricultural production and reducing losses due to biotic and abiotic stresses in the field and in storage (Sairam and Prakash, 2005; Yuan et. al., 2011). This chapter primarily deals with resistance to viral diseases. It is therefore very important that anyone embarking on a research project to genetically engineer plant...

Negative-strand RNA viruses: genetic engineering and applications.

The negative-strand RNA viruses are a broad group of animal viruses that comprise several important human pathogens, including influenza, measles, mumps, rabies, respiratory syncytial, Ebola, and hantaviruses. The development of new strategies to genetically manipulate the genomes of negative-strand RNA viruses has provided us with new tools to study the structure-function relationships of the ...

Engineering Viruses to Fight Cancer

With EMA approval in December 2015, and US FDA approval in October 2015 of an oncolytic herpesvirus for the treatment of nonresectable melanoma, we now have a novel class of anticancer agents to add to our ever-promising arsenal of cancer (immuno-) therapeutics. Talimogen laherparapvec (T-VEC) therapy involves direct, intratumoral injection of a herpes simplex type 1 (HSV-1) virus which has bee...

Genetic engineering.

Genetic engineering

BglII 5’...A_GATCT...3’ (5’overhang) BamHI 5’...G_GATCC...3’ (5’overhang); notice: MboI and Sau3AI = 5’..._GATC...3’ EcoRI 5’...G_AATTC...3’ (5’ overhang) HpaII 5’...C_CGG...3’ (5’ overhang); notice: CpG methylation blocked HindIII 5’...A_AGCTT...3’ (5’ overhang) KpnI 5’...GGTAC_C...3’ (3’ overhang) NcoI 5’...C_CATGG...3’ (5’ overhang); notice: Kozak sequence (RCC)ACCATGG NotI 5’...GC_GGCCGC......